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Blood samples were drawn from all subjects after an overnight fast and were divided into three portions: 1ml of whole blood was collected into evacuated tubes containing EDTA, for hemoglobin A1c (HbA1c) and 1ml of whole blood was collected into evacuated tubes containing potassium oxalate and sodium fluoride (2 : 1) for fasting blood glucose. Serum was separated immediately from the remaining part of the sample and stored at −20°C until analysis
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